Localization of Pf SAMS GFP BirA and biotinylated proteins in the blood stages of the Pf SAMS GFP BirA transfectant lines ..( Labeling of Pf SAMS GFP BirA in blood stage parasites Immunofluorescence assays were employed, using asexual blood stages of the Pf SAMS pfama 1 GFP BirA line and gametocytes of the Pf SAMS pffnpa GFP BirA line Mouse antibodies directed against GFP were used to detect Pf SAMS GFP BirA in schizonts ( and immature gametocytes imGC (Counterlabeling of the asexual blood stages was done with Evans Blue ( stain and of gametocytes with rabbit anti Pf s 230 antisera (The nuclei were labeled with Hoechst 33342 nuclear stain ( WT NF 54 parasites were used for negative control ..(Co localization of Pf SAMS GFP BirA and biotinylated proteins Asexual blood stages of the Pf SAMS pfama 1 GFP BirA line and gametocytes of the Pf SAMS pffnpa GFP BirA line were treated with 50 μ M biotin for 24 h and subjected to immunofluorescence assays as described above Biotinylated proteins were immunolabeled using fluorophore conjugated streptavidin ( mGC mature gametocyte TZ, trophozoite Bar 5 µm The results ( B) are representative of two independent experiments
Musabyimana JP, Distler U, Sassmannshausen J, Berks C, Manti J, Bennink S, Blaschke L, Burda PC, Flammersfeld A, Tenzer S, Ngwa CJ, Pradel G. Plasmodium falciparum S-Adenosylmethionine Synthetase Is Essential for Parasite Survival through a Complex Interaction Network with Cytoplasmic and Nuclear Proteins. Microorganisms. 2022 Jul 14;10(7):1419. doi: 10.3390/microorganisms10071419.
Other associated proteins