Pfcrk5− asexual stages grow normally and undergo gametocytogenesis. IFAs were performed on WT PfNF54 and Pfcrk5− mature stage V gametocytes thin culture smears using anti-PfP230p antisera, a marker for stage V male gametocytes (in green), in combination with anti-Pfg377 antisera, a marker for female gametocytes (in red). Representative images are shown. The parasite DNA was visualized with DAPI (blue). Scale bar = 5 μm. Merge I- merged image for red and green panels. Merge II- merged image for red, green, and DAPI (blue) channel. DIC, differential interference contrast. DAPI, 4′,6-diamidino-2-phenylindole. Symbols for male and female gametocytes are shown on left side of the image panels.

Kumar S, Gargaro OR, Kappe SHI. Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito. mBio. 2022 26:e0222722.