Expression and localization of PfCRK5 in asexual and sexual stages. (B) Immunofluorescence assays were performed on WT NF54 asexual blood stages (ring, trophozoite and schizont) to colocalize PfCRK5 (red) in combination with Histone marker H3K9Ac (green). The parasite nucleus was localized with 4′,6-diamidino-2-phenylindole (DAPI) (in blue). Scale bar = 5 μm. (D) and (E) Immunofluorescence assays were performed on stage V gametocytes using thin smears and anti-PfCRK5 antisera (in red) either in combination with α-Tubulin (marker for male gametocytes, in green) or anti- Pfg377 (marker for female gametocytes, in green). Parasite nucleus was visualized with DAPI (blue). Scale bar = 5 μm.

Kumar S, Gargaro OR, Kappe SHI. Plasmodium falciparum CRK5 Is Critical for Male Gametogenesis and Infection of the Mosquito. mBio. 2022 26:e0222722.