IFA images of PfAQP at three different asexual blood stages of Pf3D7.Methanol fixed smear of Pf3D7 infected erythro-cytes were stained with anti-PfAQP antisera fol-lowed by incubation with alexa fluor conjugated secondary antibodies (Alexa Fluor 488, green colour). DIC: differential interference contrast image, DAPI: nuclear staining using 4’,6-diamidino-2-phenylindole (blue); PfAQP: mouse anti-PfAQ Pantibodies (green); merge: overlay of PfAQP withDAPI. Scale bar 2 μm. expression of PfAQP begins at ring stage and continued to tropho-zoite and schizont stage.

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