Parasites were synchronized at the early ring stage, maintained in media as shown with 10 μM trimethoprim (+TMP) or an equal volume of DMSO (-TMP), or 100 nM aTc (+aTc) or an equal volume of DMSO (-aTc). Parasites were fixed 24 hours after invasion as trophozoites and stained to compare protein export in Hsp101-DD +/- TMP and 1437APT +/- aTc. Exported proteins are marked by anti-HRP2 or anti-FIKK4.2, each shown in green; the parasite cytosol is marked by anti-aldolase shown in red; parasite nucleus marked by DAPI in blue. Experiment was performed three times; representative images shown. Scale bars represent 5 μm.
Polino AJ, Hasan MM, Floyd K, Avila-Cruz Y, Yang Y, Goldberg DE. An essential endoplasmic reticulum-resident N-acetyltransferase ortholog in Plasmodium falciparum. J Cell Sci. 2023 Mar 15;136(6):jcs260551.
Other associated proteins
|PF3D7_0831800||histidine-rich protein II|
|PF3D7_1116800||heat shock protein 101 chaperone protein ClpB2|