Pf3D7-1437000 depletion does not affect protein export. (A) Parasites were synchronized at the early ring stage, maintained in medium as shown with 10 μM trimethoprim (+TMP) or an equal volume of DMSO (−TMP), or 100 nM aTc (+aTc) or an equal volume of DMSO (−aTc). Parasites were fixed 24 h after invasion as trophozoites and stained to compare protein export in Hsp101-DD +/−TMP and 1437APT +/−aTc. Exported proteins are marked by antiHRP2 or anti-FIKK4.2, each shown in green; the parasite cytosol is marked by anti-aldolase, shown in red; parasite nucleus marked by DAPI is in blue. Experiment was performed three times; representative images are shown. Scale bars: 5 μm.
Polino AJ, Hasan MM, Floyd K, Avila-Cruz Y, Yang Y, Goldberg DE. An essential endoplasmic reticulum-resident N-acetyltransferase ortholog in Plasmodium falciparum. J Cell Sci. 2023 136(6):jcs260551. PMID: 36744402;
Other associated proteins
|PF3D7_0831800||histidine-rich protein II|
|PF3D7_1116800||heat shock protein 101 chaperone protein ClpB2|
|PF3D7_1443800||pre-mrna-splicing factor cwc24|