Subcellular localization of ARMA and induction of functional antibodies. Immunofluorescent staining showing the colocalization of ARMA with MSP1 on the merozoite surface. Late schizont stages were double stained with rabbit antisera (anti-V1 or anti-V2) and mouse anti-MSP1 (full-length) in immunofluorescence assay. Rabbit preimmune sera were used to assess non-specific binding. The merozoite nuclei were stained with Hoechst. Incubation of parasites with only anti-rabbit antibodies excluded non-specific binding of secondary reagents.

Niaré K, Chege T, Rosenkranz M, Mwai K, Saßmannshausen Z, Odera D, Nyamako L, Tuju J, Alfred T, Waitumbi JN, Ogutu B, Sirima SB, Awandare G, Kouriba B, Rayner JC, Osier FHA. Characterization of a novel Plasmodium falciparum merozoite surface antigen and potential vaccine target. Front Immunol. 2023 14:1156806. PMID: 37122725;