Mutations of phosphorylation sites do not affect the localization of GFP-tagged PfGAP40 proteins to the parasite periphery. IFA analysis of matured schizonts and merozoites from three GFP::PfGAP40 mutants (GFP::WT, GFP::3S-to-D, and GFP::3S-to-A). Specific GFP-2×FKBP-PfGAP40 fusion proteins were probed with anti-PfGAP45 (green) and anti-GFP (red) antibodies. BF, bright field. Nuclei are stained with DAPI (blue). Scale bar: 5 μm.

He L, Qiu Y, Pang G, Li S, Wang J, Feng Y, Chen L, Zhu L, Liu Y, Cui L, Cao Y, Zhu X. Plasmodium falciparum GAP40 Plays an Essential Role in Merozoite Invasion and Gametocytogenesis. Microbiol Spectr. 2023 May 30:e0143423.