Indirect immunofluorescence assay showed that there was a significant reduction in the colocalization (yellow) of Cytc (green) and PfTopoVIB/ PfSpo11 (red), respectively, in the parasites treated with 0.5 nM atovaquone compared to the untreated parasites. The mean PCC values were determined from 25 individual cells from each of the IFA, and the average value is presented at the bottom of merged image.

Singh P, Tabassum W, Fangaria N, Dey S, Padhi S, Bhattacharyya MK, Arun Kumar K, Roy A, Bhattacharyya S. Plasmodium Topoisomerase VIB and Spo11 Constitute Functional Type IIB Topoisomerase in Malaria Parasite: Its Possible Role in Mitochondrial DNA Segregation. Microbiol Spectr. 2023 11(3):e0498022.