PI-PLC has an essential role in trophozoite and schizont development. Live cell microscopy of ring (R), trophozoite (T), early schizont (ES), and late schizont (LS) stages of PI-PLC-GFP-KS parasites expressing endogenously FKBP-GFP-tagged PI-PLC (green) in addition to a nuclear mislocalizer (rapamycin) fused to mCherry (NLS-ML, magenta). Parasites were either untreated (control) or treated with Rapa to conditionally mislocalize the PI-PLC to the nucleus. DIC, differential interference contrast. Scale bars, 5 µm.

Burda PC, Ramaprasad A, Bielfeld S, Pietsch E, Woitalla A, Söhnchen C, Singh MN, Strauss J, Sait A, Collinson LM, Schwudke D, Blackman MJ, Gilberger TW. Global analysis of putative phospholipases in Plasmodium falciparum reveals an essential role of the phosphoinositide-specific phospholipase C in parasite maturation. mBio. 2023 25:e0141323.