Identification of PhIL1-interacting candidates using BioID. (a) IMC localisation ofPfPhIL1-BirA-GFP in schizonts and merozoites.Nuclei were stained with Hoechst-33,342. Scale bar, 2μm. (b)PfPhIL1-based biotinylation at the IMC.PfPhIL1-BirA-GFP (mouse anti-GFP, green)or 3D7 control parasites were fixed with MeOH at the schizont stage and biotinylated proteins were visualized by streptavidin-594 (red).Hoechst was used for DNA staining (blue). Scale bar, 1μm.