PF3D7_0831700 PfHsp70-x

Targeting of PfHsp70-x to the host cell is dependent on an eight-amino-acid sequence. A. Structure of N-terminal region of PfHsp70-x. SP, predicted signal peptide cleavage site. Numbering refers to amino acids from N-terminus. The linker region between putative signal sequence cleavage site and the start of the ATPase domain is shown in dark blue. B. Live cell imaging of parasites expressing various N-terminal GFP fusion chimera. Numbers refer to amino acids from N-terminus. In merge and overlay: green, GFP; blue, Hoechst. Chimera containing up to 25AA show fluorescence exclusively in the PV, whereas chimera containing more than 32AA also show fluorescence in the host cell cytosol.

K├╝lzer S, Charnaud S, Dagan T, Riedel J, Mandal P, Pesce ER, Blatch GL, Crabb BS, Gilson PR, Przyborski JM. Plasmodium falciparum-encoded exported hsp70/hsp40 chaperone/co-chaperone complexes within the host erythrocyte. Cell Microbiol. 2012 Aug 1. [Epub ahead of print]