Cytolocalization of P. falciparum folate transporters PfFT1 and PfFT2. A, fluorescence signals given by P. falciparum transformed with a PfFT1-GFP C-terminal fusion construct are shown. A trophozoite (top panels) shows a signal that coincides with the parasites plasma membrane. Dividing stages of early and late schizonts are in the two lower panels. In the schizonts the labeling of merozoite plasma membranes is apparent. B, indirect immunofluorescence of PfFT2 is shown. Permeabilized cells were labeled with an anti-PfFT2 antibody. Again trophozoites (upper and middle panels) as well as merozoites in the schizont in the lower panel presented strong plasma membrane signal. Bars represent 2mm.

Salcedo-Sora JE, Ochong E, Beveridge S, Johnson D, Nzila A, Biagini GA, Stocks PA, O'Neill PM, Krishna S, Bray PG, Ward SA. The molecular basis of folate salvage in Plasmodium falciparum: characterization of two folate transporters. J Biol Chem. 2011286(52):44659-68.