Localization of PfRH2a/b by immunofluorescence confocal microscopy. (A) 3D7 schizonts were dual labeled with anti-rPfRH240 mice sera and anti-clag3.1 rabbit sera. Mature schizonts immunolabeled with anti-rPfRH240 were stained with Alexa 488 linked anti-mouse IgG secondary antibody (green). Schizonts labeled with anti-clag3.1 rabbit sera were stained with Alexa 594 linked anti-rabbit IgG secondary antibody (red). (B) 3D7 mature schizonts were dual labeled with anti-rPfRH240 mouse sera and anti-EBA175 rabbit sera. Schizonts labeled with anti-EBA-175 antibodies were stained with Alexa 594 linked anti-rabbit IgG secondary antibody (red). PfRH2a/b co-localizes with the known rhoptry bulb marker protein, clag3.1 demonstrating that it is localized in the rhoptries, and not with the microneme marker protein, EBA-175.
Sahar T, Reddy KS, Bharadwaj M, Pandey AK, Singh S, Chitnis CE, Gaur D. Plasmodium falciparum reticulocyte binding-like homologue protein 2 (PfRH2) is a key adhesive molecule involved in erythrocyte invasion. PLoS One. 2011 6(2):e17102.
Other associated proteins
|PF3D7_0302500||cytoadherence linked asexual protein 3.1|
|PF3D7_0731500||erythrocyte binding antigen-175|