GFP targeting by P. falciparum thioredoxin 2. (A)–(C) Targeting of Trx2 to the parasitophorous vacuole and to a not yet characterized, non-dividing organelle within the parasite. Colocalization of GFP with the mitochondrial stain MitoTrackerOrange in fixed cells. Colocalization of GFP and the apicoplast marker ACP in fixed, immunodecorated cells. Trx2 is clearly localized in the parasitophorous vacuole. Trx2-GFP labeled further structures within the parasite (B, C) in .90% of the parasites. In these parasites, the GFP fluorescence did neither colocalize with the ER nor with the nucleus, the mitochondrion or the apicoplast, as evidenced by analysis using organellar markers.
Kehr S, Sturm N, Rahlfs S, Przyborski JM, Becker K. Compartmentation of redox metabolism in malaria parasites. PLoS Pathog. 2010 6:e1001242.