PF3D7_0316900 E3 ubiquitin-protein ligase, putative

Ubiquitylation proteins localize to the apicoplast in Plasmodium falciparum. Immunofluorescence assays detecting the respective ubiquitylation factor

indicated (white lettering) in the left-most panel. Proteins were detected by tagging with an HA or GFP epitope in the genomic locus (C, D), by ectopic fusion with partial coding sequence (E), or by using an antibody raised again recombinant protein (C). Staining for the apicoplast markers Cpn60 and ACP is shown in the second lane. Merged images also show DAPI staining for P. falciparum

Agrawal S, Chung DW, Ponts N, van Dooren GG, Prudhomme J, Brooks CF, Rodrigues EM, Tan JC, Ferdig MT, Striepen B, Le Roch KG. An Apicoplast Localized Ubiquitylation System Is Required for the Import of Nuclear-encoded Plastid Proteins. PLoS Pathog. 2013 9(6):e1003426

Other associated proteins

PFID Formal Annotation
PF3D7_0208500 acyl carrier protein
PF3D7_0312100 E3 ubiquitin-protein ligase, putative
PF3D7_1345500 ubiquitin-conjugating enzyme E2