N-terminal domain of the PfPrp16p targets the protein to the nucleus. (A) Schematic representation of the PfPrp16-GFP chimeric constructs. Predicted nuclear localization sequence, “KRRK,” is shown as a black box at 41st–44th amino acid position. (B and C) Immunofluorescence assay based localization of the chimeric GFP proteins at (B) trophozoite and (C) schizont stages respectively. Anti-GFP (red, 1:200 dilution) and anti-PfTSN (green, 1:100 dilution) antibodies were used on thin blood smears of parasite lines. PfPrp16.1-40-GFP and PfPrp16.81-last-GFP chimeric proteins do not localize with the nuclear marker PfTSN protein in either of the developmental stages, whereas chimeric protein PfPrp16.1-80-GFP was trafficked to the nucleus in trophozoite and schizont stages.
Singh PK, Kanodia S, Dandin CJ, Vijayraghavan U, Malhotra P. Plasmodium falciparum Prp16 homologue and its role in splicing. Biochim Biophys Acta. 2012 1819(11-12):1186-99
Other associated proteins
|PF3D7_1364300||pre-mRNA-splicing factor ATP-dependent RNA helicase PRP16|