Localization of native, functional PfFC in the parasite by immunofluorescence 304 microscopy. Mouse anti-rPfFC IgG, rabbit anti-r(D)PfPBGD (apicoplast marker) IgG and rabbit anti305 rPfHSP60 (mitochondrial marker) IgG were used at 1:200 dilution. The slides were examined using a 306 Fluorescence Microscope (Leica Microsystems) and the images were acquired at 100x 307 magnification using an oil immersion objective and Leica FW 4000 image acquisition 308 software. IE, infected erythrocyte; P, parasite and H, hemozoin. Scale bar = 5 mm.

Nagaraj VA, Prasad D, Rangarajan PN, Padmanaban G. Mitochondrial localization of functional ferrochelatase from Plasmodium falciparum. Mol Biochem Parasitol. 2009 168:109-12. Copyright Elsevier 2010