PfHsp110c prevents the aggregation of Asn-rich protein during febrile episodes. Live fluorescence images of PfHsp110c–RFA parasite lines expressing (a,b) the Asn repeat-rich parasite protein, PFI1155w. incubated at 400C for 6 h with (a) or without (b) 10mM TMP. The arrowheads indicate fluorescent foci of PFI1155w–tagRFP-T (b). Scale bar represents 2 mm. Images from left to right are phase, DAPI, GFP (MAL13P1.540), RFP (PFI1155w) and GFP–RFP merge. Parasites that were subjected to heat shock without TMP showed foci of PFI1155w–tagRFP-T (b) and indicative of protein. The distribution of PfHsp110c–RFA also was more focal upon heat shock in the absence of TMP (b) in the absence of TMP is specifically owing to the loss of PfHsp110c function.

Muralidharan V, Oksman A, Pal P, Lindquist S, Goldberg DE. Plasmodium falciparum heat shock protein 110 stabilizes the asparagine repeat-rich parasite proteome during malarial fevers. Nat Commun. 2012 Dec 18;3:1310.