PF3D7_0800200 erythrocyte membrane protein 1, PfEMP1

Immunofluorescence analysis of VARC in mature trophozoites. Methanol-fixed parasitized erythrocytes reacted with mouse anti-VARC antibody (1:1000), fluorescein isothiocyanate-conjugated anti-mouse antibodies (1:2000), and DAPI (0.1 mM). The labeled parasites were photographed using a fluorescence microscope (Nikon) at x40 magnification. The left panel shows fluorescence forVARC (green), counterstained for nuclei with DAPI (blue); the right panel shows the corresponding bright field + DAPI.

Hora R, Bridges DJ, Craig A, Sharma A. Erythrocytic casein kinase II regulates cytoadherence of Plasmodium falciparum-infected red blood cells. J Biol Chem. 2009 284:6260-9.

img