PF3D7_0917900 PfHsp70-2

Sub-cellular localization of P. falciparum DRM-associated proteins. Immunofluorescence studies were performed on paraformaldehyde-fixed P.falciparum trophozoite-iRBCs using mouse immune sera raised against the DRM-associated proteins PFJ130, (PF10_0130) PFN201, (PF14_0201 surface protein) PFB985c, (PFB0985c) Pfmc-2TM Maurer's cleft two transmembrane protein; PFL1070c, endoplasmin homolog precursor) PFK351 (PF11_0351) HSP70. Immune sera specific for PfBip and PfEXP1-were used for co-labeling, as indicated. Mitochondria labeling was performed with MitoTracker® probe. Parasite nuclei were stained with Hoechst 33342. Microscopic observations were made on an apotome-equipped Axio Imager 2 (Carl Ziess, Jena) with a 100× apochromat objective and DIC. The differential interference contrast (DIC), merged and overlay images are presented. Bar size: 5 µm. PfK351overlays mitotracker thus localized to parasite mitochondrion. PfL1070 colocalizes with the ER-resident marker PfBiP. PfK55, a conserved hypothetical protein with unknown function, contains a SP and a ER retention signal (KDEL), but due to poor IFA, the exact subcellular localization of this protein remained elusive.

Yam XY, Birago C, Fratini F, Di Girolamo F, Raggi C, Sargiacomo M, Bachi A, Berry L, Fall G, Curra C, Pizzi E, Braun-Breton C, Ponzi M. Proteomic analysis of detergent-resistant membrane microdomains in trophozoite blood stage of the human malaria parasite Plasmodium falciparum. Mol Cell Proteomics. 2013 12(12):3948-61

Other associated proteins

PFID Formal Annotation
PF3D7_0222100 Pfmc-2TM Maurer's cleft two transmembrane protein
PF3D7_1013300 conserved Plasmodium protein, unknown function
PF3D7_1121600 parasitophorous vacuole membrane antigen QF 116 exported protein 1 circumsporozoite-related antigen
PF3D7_1134000 PfHsp70-3
PF3D7_1222300 endoplasmin, putative
PF3D7_1420700 surface protein P113