Post-embedding immunolabeling of FC27 with human antl-Ag 23 and protein A-gold (bars equal 0.5 mm) Samples were fixed for 10 rain with 0.25% glutaraldehyde and embedded m L.R. White resin, heat polymerized at 50°C. (A) Trophozoite-infected erythrocyte with labeling evenly dispersed over the cytoplasm. (B) Mature trophozoite-infected cell with more labeling over the erythrocyte cytoplasm and over a presumed food vacuole (arrow). Antigen is apparently leaking from an area of damaged erythrocyte plasma membrane (arrow head) (C) Schizont-infected erythrocyte containing developing merozoites. Label is again over the erythrocyte cytoplasm largely exterior to the presumed parasitophorous vacuole membrane. (D) Uninfected erythrocytes without significant labeling.
Bianco AE, Culvenor JG, Coppel RL, Crewther PE, McIntyre P, Favaloro JM, Brown GV, Kemp DJ, Anders RF. Putative glycophorin-binding protein is secreted from schizonts of Plasmodium falciparum. Mol Biochem Parasitol. 1987 23:91-102. Copyright Elsevier 2010.