RON12 is predominantly secreted late into the nascent PV. Immunofluorescence and DIC images of merozoites fixed during invasion

of erythrocytes. From left to right anti-RON12 (green), anti-RON4 depicting the moving junction (red), overlay of both with nuclear stain DAPI, DIC image and overlay of all images. Cartoon schematic is shown on the right of each panel. Arrowheads depict location of moving junction. Size bars equal 1 μm.

Knuepfer E, Suleyman O, Dluzewski AR, Straschil U, O'Keeffe AH, Ogun SA, Green JL, Grainger M, Tewari R, Holder AA. RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation. Cell Microbiol. 2013 Aug 12. [Epub ahead of print]