RON12 is located within the rhoptry neck.. A and B. Immunofluorescence and bright-field images of schizonts (A) and merozoites (B). Images from left to right show anti-RON12 labelling (green) followed by microneme (anti-AMA1), rhoptry (neck: anti-RON4; body: anti-RAMA) and surface (anti-MSP119) specific antibodies in red, overlay of both with DAPI-stained nuclei and the bright-field image. Size bars in (A) equal 2 μm and in (B) 1 μm. C. Immuno-electron microscopy of isolated merozoites. Localization of RON12 in the rhoptry neck of three different merozoites is shown. Size bars equal 100 nm.

Knuepfer E, Suleyman O, Dluzewski AR, Straschil U, O'Keeffe AH, Ogun SA, Green JL, Grainger M, Tewari R, Holder AA. RON12, a novel Plasmodium-specific rhoptry neck protein important for parasite proliferation. Cell Microbiol. 2013 Aug 12. [Epub ahead of print]