Identification of two novel P. falciparum nucleolar proteins. (a) IFA colocalisation of NuProC2-3xHA and NuProC3-2xTy in a double transgenic line. Epitope-tagged proteins were detected using anti-HA (red) and anti-Ty (green) antibodies. (b) NuProC2-3xHA and NuProC3-3xHA co-localize with nucleolar fibrillarin. HA-tagged proteins were detected in single transgenic lines using anti-HA antibodies (red). Fibrillarin (PfFIB) was visualized using an anti-human fibrillarin antibody.

Oehring SC, Woodcroft BJ, Moes S, Wetzel J, Dietz O, Pulfer A, Dekiwadia C, Maeser P, Flueck C, Witmer K, Brancucci NM, Niederwieser I, Jenoe P, Ralph SA, Voss TS. Organellar proteomics reveals hundreds of novel nuclear proteins in the malaria parasite Plasmodium falciparum. Genome Biol. 2012 Nov 26;13(11):R108.