A. Endogenously tagging nPrx confirms its status as a nuclear protein. Epifluorescence microscopy on the 3D7nPrx-3’GFP line demonstrates that the tagged nPrx localises to the nucleus in all blood stages.

B. PfnPrx is a nuclear protein. Immunoelectron microscopy using the rabbit anti-PfnPrx antibody on schizont stage parasites reveals that PfnPrx is in the electron dense nuclear periphery. The image shows a single merozoite located within a schizont.

Richard D, Bartfai R, Volz J, Ralph SA, Muller S, Stunnenberg HG, Cowman AF. A genome-wide chromatin-associated nuclear from the malaria parasite Plasmodium falciparum. J Biol Chem. 2011 Jan 31. [Epub ahead of print]