(A) Immunofluorescence of early schizont parasites indicated that H103 partially co-localises with EBA175 (i) and AMA1 (ii) in the micronemes. DAPI staining indicates that the parasites are early schizonts.

(B) Immunofluorescence of late stage schizont parasites using antibodies to H103, EBA175 (i), RAP1 (ii) and MSP4 (iii) indicated that at this stage of the asexual cycle H103 was located on the merozoite surface.

H103 did not co-localise with the microneme protein EBA175 or the rhoptries protein RAP1 at this stage of the cycle. DAPI staining shows the location of DNA and signifies that the parasites are fully segmented schizonts.

Pearce JA, Mills K, Triglia T, Cowman AF, Anders RF. Characterisation of two novel proteins from the asexual stage of Plasmodium falciparum, H101 and H103. Mol Biochem Parasitol. 2005 139:141-51. Copyright Elsevier 2009.