Immunofluorescence assay of rabbit antisera against PfMC-2TM peptides colocalized with Mab SP1C1. Immunofluorescence assays (IFA) in this figure and Figs. 4, 5, 6, 7, 8, and 9 were performed using the same stage of parasites. Trophozoite- and schizont-infected erythrocytes were incubated with mouse and rabbit primary antibodies, followed by secondary antibodies directed to both species, conjugated to different colored fluorochromes; Alexa 488 and Alexa 568 (Molecular Probes) for detection of primary antibodies. Antibody staining was considered colocalized if there was overlap (yellow/orange) in the staining pattern obtained with the two antibodies on or within the same structure or within domains of the intra-membranous network.

Tsarukyanova I, Drazba JA, Fujioka H, Yadav SP, Sam-Yellowe TY. Proteins of the Plasmodium falciparum two transmembrane Maurer's cleft protein family, PfMC-2TM, and the 130 kDa Maurer's cleft protein define different domains of the infected erythrocyte intramembranous network. Parasitol Res. 2009 104:875-91. Copyright Springer 2011.