STEVOR-specific immunofluorescence staining of mature (>24 h after red blood cell invasion) blood-stage P. falciparum D10 (A and C) and representative field isolates K1657 and K1640 from patients in Kilifi, Kenya (B and D), respectively. Parasites were stained using anti-STEVOR peptide 1 antibodies (A and B) or anti-S1 serum (C and D). Early trophozoite (ET), late trophozoite (LT), and schizont (S) stage parasites are shown. All samples were stained with either rabbit anti-STEVOR peptide 1 affinity-purified antibodies (Stevor) or with anti-S1 rabbit serum (STEVOR), as well as the Maurer’s cleft specific anti-PfSBP1 mouse serum (PfSBP1) and the DNA-specific nuclear stain DAPI (at 1 mg/ml). The individual stains and the merged image (Merge) are shown. STEVOR antibody gave a punctate staining pattern throughout the iRBC, present from the late trophozoite stage and throughout early/mid-stage schizont development in 3D7, D10, and K1657 However, in the highly developed segmented schizont, the staining pattern of STEVOR was more diffuse both around the parasite nuclei and throughout the iRBC cytosol, with some potentially associated with the iRBC surface membrane.
Blythe JE, Yam XY, Kuss C, Bozdech Z, Holder AA, Marsh K, Langhorne J, Preiser PR. Plasmodium falciparum STEVOR proteins are highly expressed in patient isolates and located in the surface membranes of infected red blood cells and the apical tips of merozoites. Infect Immun. 2008 76(7):3329-36.
Other associated proteins
|PF3D7_1040200||PIR protein stevor|