Localization of PfMLH immunofluorescence staining. The cells were fixed and immunostained. Panel (i) super-imposed image of phase and DAPI, (ii) immunofluorescently stained cell (green, P. falciparum MLH), (iii) image of cell stained with DAPI, (iv) and (v) super-imposed images. Panel “a” is the schizont stage stained with pre-immune IgG and panel “b–e” are ring, trophozoite, schizont and merozoite stages of the development of P. falciparum 3D7 strain stained with immune IgG. Panel “f” is schizont stage of the development of P. falciparum Dd2 strain stained with immune IgG. PfMLH is mainly localized in the nuclear compartment. there was no detectable expression of PfMLH in the ring and trophozoite stages.

Tarique M, Satsangi AT, Ahmad M, Singh S, Tuteja R. Plasmodium falciparum MLH is schizont stage specific endonuclease. Mol Biochem Parasitol. 2011 181(2):153-61