Expression and localization of the H-protein in H-proteinDd2 cells. Co-localization of GFP fluorescence from H-proteinDd2 cells with mitotracker and Hoechst dyes. Live cells from the H-proteinDd2 cell line were incubated with 100 nM mitotracker CMX-Ros (Invitrogen) and 1 mg/ml Hoechst stain for 20 min at 37 ◦C followed by 2 washout periods in complete media for 5 min. Cells were imaged on a Nikon Eclipse E800 epifluorescent microscope, and the images were processed with ImageJ software. The H-protein is localized exclusively to the mitochondrion.

Spalding MD, Allary M, Gallagher JR, Prigge ST. Validation of a modified method for Bxb1 mycobacteriophage integrase-mediated recombination in Plasmodium falciparum by localization of the H-protein of the glycine cleavage complex to the mitochondrion. Mol Biochem Parasitol. 2010 172(2):156-60. Copyright Elsevier