Labeling of Fal2-S2 transfectants with BODIPY-TR ceramide (BPY) at trophozoite stage. BODIPY-TR labels the erythrocyte membrane, PVM and food vacuole membrane. GFP fluorescence is seen overlapping with fluorescence by BODIPY-TR in the parasitophorous vacuole (arrowhead), trans vesicular network (panel II, pointed arrow) and in the food vacuolei
Dasaradhi PV, Korde R, Thompson JK, Tanwar C, Nag TC, Chauhan VS, Cowman AF, Mohmmed A, Malhotra P. Food vacuole targeting and trafficking of falcipain-2, an important cysteine protease of human malaria parasite Plasmodium falciparum. Mol Biochem Parasitol. 2007 156:12-23. Copyright Elsevier 2009.