HSP101 and PTEX150 co-localize and have dual apical merozoite and PVM localization. Double labelling IFA on fixed 3D7-150HA ring- and merozoite-stage parasites using the antibodies as indicated. 3D7-150HA is a transgenic P. falciparum (3D7) parasite line where the endogenous gene was modified at its carboxy terminus to include a triple haemagglutinin (HA) epitope tag. The arrows indicate the apical end of the merozoite. BF, bright-field; DAPI, nucleic acid stain 49,6-diamidino-2-phenylindole. PTEX150 and HSP101 co-localize and are found in discrete foci in the membranes surrounding the ring-stage parasite. This membrane is the PVM, as fluorescence surrounds the parasite membrane marker MSP1 (PFI1475w).
de Koning-Ward TF, Gilson PR, Boddey JA, Rug M, Smith BJ, Papenfuss AT, Sanders PR, Lundie RJ, Maier AG, Cowman AF, Crabb BS. A newly discovered protein export machine in malaria parasites. Nature. 2009 459(7249):945-9.
Other associated proteins
|PF3D7_1116800||heat shock protein 101 chaperone protein ClpB2|