Intracellular localisation of PfTKL2 by immunofluorescence assay of wild type 3D7 parasites. (a) Co-localisation of PfTKL2 and the parasitophorous vacuole protein Exp1. (b) Co-localisation of PfTKL2 and the red blood cell cytoskeleton protein spectrin. DAPI was used to stain the parasite DNA. Scale bar is 2 μm. Co-localisation experiment using a parasitophorous vacuole marker (Exp1) clearly shows that a fraction of the PfTKL2 protein is exported into the RBC cytoplasm (a). Further, the co-localisation of PfTKL2 with RBC spectrin shows that PfTKL2 is present in the RBC cytosol.

Abdi AI, Carvalho TG, Wilkes JM, Doerig C. A secreted Plasmodium falciparum kinase reveals a signature motif for classification of tyrosine kinase-like kinases. Microbiology. 2013 159(Pt 12):2533-47