PF3D7_1121600 parasitophorous vacuole membrane antigen QF 116 exported protein 1 circumsporozoite-related antigen

Confocal fluorescence microscopy images of transfected 3D7 P. falciparum-infected RBCs expressing a GFP chimera of a fragment of Exp-1 that is directed to the parasitophorous vacuole. DIC image, the GFP fluorescence signal and an overlay of Exported protein-11-35-GFP transfectant. Scale bar = 5 µm.

Top and middle rows show trophozoite stage parasite. The top cell shows extensions of the parasitophorous vacuole to form the tubulovesicular network (TVN). The middle cell shows a necklace of beads pattern in parasitophorous vacuole. In the schizont stage parasite (bottom row) the protein accumulates around the individual merozoites forming a segmented/ wagon wheel pattern around a fluorescent residual body.

Tilley L, McFadden G, Cowman A, Klonis N. Illuminating Plasmodium falciparum-infected red blood cells. Trends Parasitol. 2007 23:268-77. Copyright Elsevier

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