GFP expressed in transfectants of P. falciparum. RBC infected by the transfectant with pSSPF2/PfACP-GFP (A and B), pSSPF2/PfHsp60-GFP (C and D) or pSSPF2/PfIscS-GFP (E and F) were incubated in RPMI 1640 medium containing Hoechst 33342 (HT: blue) at 1 mg/ml and MitoTracker Red CM-H2XRos (MTR: red) at 100nM (A, C, E) or 10nM (B, D, F) to stain the nucleus and mitochondrion, respectively. Localization of GFP (green) in the transfectant parasites in the trophozoite (A, C, E) or schizont (B, D, F) stage was monitored by fluorescence microscopy using a DeltaVison microscope system (Applied Precision). +phase: GFP + MTR + HT superimposed on a phase contrast image. Scale bar: 5 mm. Both Hsp60 and IscS target to the mitochondrion.
Sato S, Rangachari K, Wilson RJ. Targeting GFP to the malarial mitochondrion. Mol Biochem Parasitol. 2003 130:155-8.
Other associated proteins
|PF3D7_0727200||cysteine desulfurase ISCS|