Single and dual immunofluorescent analysis of the subcellular localization of AMA-1, RAP-l and MSPl in mature schizonts, free merozoites, and ring stages of P. falciparum 7G8. Descriptors of the subcellular localization follow. Co-localized apical immunofluorescence of C-terminal AMA-1 and RAP-l in mature schizont, (A and D, respectively). Co-localized apical immunofluorescence of C-terminus-AMA-1 (x) and RAP-l in free merozoite, (B and E, respectively); surface localization of C-terminus-AMA-1 (y) and apical localization of RAP-l in free merozoite, (B and E, respectively). Co-localized apical immunofluorescence of N-termini-AMA-1 and RAP-l in mature schizont and free merozoite, (C, F and G, H, respectively). Surface localization of MSPl in mature schizont (I). Co-localization of C-terminus-AMA-1 and RAP-l in ring stage parasite, (J and K, respectively). Methanol-fixed schizont/ring stage thin films (ring development less than 3 h) were reacted with rat mAb IgG (25 pg ml-1), or rat mAb IgG and RAP-l culture supematant (dil. 15) in phosphate-buffered saline containing 1% foetal calf serum. Secondary stains were tetramethyl rhodamine isothiocyanate coupled or fluorescein isothiocyanate coupled anti-rat (A,B,C,G,J or I, respectively) and FITC coupled anti-mouse (human and rat adsorbed) (D,E,F,G and K).

Narum DL, Thomas AW. Differential localization of full-length and processed forms of PF83/AMA-1 an apical membrane antigen of Plasmodium falciparum merozoites. Mol Biochem Parasitol. 1994 67:59-68. Copyright Elsevier 2009.