Expression of the PfPrp16p during asexual blood stages of P. falciparum. Immunofluorescence assay based localization of the PfPrp16p. Anti-PfPrp16p sera (1:200 dilution) were used to localized the protein using thin blood smears of P. falciparum ring, trophozoite and schizont stages. DAPI and anti-Pf‐Tudor-SN (PfTSN) antibody were used to localize the nucleus. PfTSN co-localizes with Pf-Tudor-SN (PfTSN), a nuclear resident protein. At the trophozoite and schizont stages the protein was detected in the nucleus as well as in the cytoplasm indicating nucleo-cytoplasmic shuttling of PfPrp16p.

Singh PK, Kanodia S, Dandin CJ, Vijayraghavan U, Malhotra P. Plasmodium falciparum Prp16 homologue and its role in splicing. Biochim Biophys Acta. 2012 1819(11-12):1186-99