Localization of PfSUB2 protein by immunofluorescence on segmented schizonts and by IEM on fixed merozoites. (A and B) An air-dried fixed segmented schizont incubated with 25 mgyml of propidium iodide to label the parasite nuclei (A) and mouse anti-GSTPfSUB2 antibodies (B). Bound antibodies then were detected with FITC-coupled anti-mouse antibodies (magnification: 31,500). (C) The IEM analysis of thin sections of resin-embedded merozoites probed with mouse anti-GST-PfSUB2 antibodies and revealed with gold-labeled anti-mouse IgG antibody. M, mitochondrion.
Barale JC, Blisnick T, Fujioka H, Alzari PM, Aikawa M, Braun-Breton C, Langsley G. Plasmodium falciparum subtilisin-like protease 2, a merozoite candidate for the merozoite surface protein 1-42 maturase. Proc Natl Acad Sci U S A. 1999 96:6445-50. Copyright 2009 National Academy of Sciences, U.S.A