Wide field IFA time course of invasion using anti-HA(PfSUB2HA)/PfRON4. PfSUB2, is a micronemal protease, sheddase, responsible for MSP1 release. Scale bar = 2.0 μm. In parasites at early stages of invasion, the majority of PfSUB2 had reached the posterior pole of the merozoite (PfSUB2 early). Although not colocalized in the same parasite, the lack of contiguity between shed MSP1 and PfSUB2 sheddase, each in comparison with PfRON4 (PfSUB2 mid), suggests that MSP1 may be processed by PfSUB2 early in invasion but shed only during passage through the junction. In the latter stages of invasion, PfSUB2 remained at the posterior pole (SUB2 late).

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