The cellular localization of PfNF-YB was examined in ring, trophozoite and schizont stages of intra-erythrocytic cycle. PfNF-YB was localized at both cytoplasm and nucleus. Immunofluorescence was carried out with the anti-PfNF-YB antibody (green), and nuclear DNA was stained with TOPRO-3 (blue) and the RBC membrane bordering with WGA (red). The confocal images exhibited a specific staining to parasites and displayed localization in both cytoplasm and nucleus in all stages. Scale bar = 5 mm.
Lima WR, Moraes M, Alves E, Azevedo MF, Passos DO, Garcia CR. The PfNF-YB transcription factor is a downstream target of melatonin and cAMP signalling in the human malaria parasite Plasmodium falciparum. J Pineal Res. 2012 54(2):145-53