Immunofluorescence assay in paraformaldehyde fixed ring-stage parasites with anti-PfSir2a antibodies (red) in wild-type parasites (first row) and anti-mCherry antibodies (red) in 3D7DSir2aþSir2aHmCherry parasites (second row). Nuclei of ring-stage parasites were stained with DAPI (blue). Scale bar, 1 mm. PfSir2aHmCherry was enriched at the nuclear periphery, forming foci that resemble the perinuclear pattern (telomeric clusters and nucleolus) previously shown for the native PfSir2a.
Mancio-Silva L, Lopez-Rubio JJ, Claes A, Scherf A. Sir2a regulates rDNA transcription and multiplication rate in the human malaria parasite Plasmodium falciparum. Nat Commun. 2013 4:1530.