Colocalization of PfMSRP-1 and PfMSRP-2 with P. falciparum MSP-1 as demonstrated by immunofluorescence of thin blood smears of asynchronous cultures. (a) Slides were incubated with a mouse anti-PfMSRP-1 serum and a fluorescein isothiocyanate-labeled rabbit anti-mouse IgG secondary antibody. (b and f) Slides were incubated with a guinea pig anti-Pf83a serum and a rhodamine-labeled donkey anti-guinea pig IgG secondary antibody. (c) Overlay of panels a and b. (d) Bright-field image of panels a, b, and c. (e) Slides were incubated with a mouse anti-PfMSRP-2 serum and a fluorescein isothiocyanate-labeled rabbit anti-mouse IgG secondary antibody. (g) Overlay of panels e and f. (h) Bright-field image of panels e, f, and g. PfMSRP-1 and PfMSRP-2 were localized to the membranes of trophozoites (a and e). This is similar to the pattern observed with the amino-terminal portion of PfMSP-1 (b and f).

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