PF3D7_1335100 merozoite surface protein 7

MSP7 co-localizes with MSP1 PFI1475w on mature blood stages and merozoites of P. falciparum. Acetone–methanol fixed mature P. falciparum T9/96 schizonts were analysed by IFA and visualised with mouse anti-MSP7A (MSP7A) and rabbit anti-MSP1 (MSP1) antibodies. DAPI was used to stain nuclei. A merge of anti-MSP7 and anti-MSP1, and of anti-MSP7 and anti-MSP1 and DAPI are shown. (D) Transmission electron micrograph showing MSP7-specific immuno-gold staining of the surface of (a) developing merozoites within the schizont and (b) a free merozoite; (c) double immuno-gold staining of the surface of a free merozoite labelled for MSP7 (the smaller, 5 nm gold particles) and MSP1 (the larger, 10 nm gold particles). The inset in (c) shows double labelling of part of the surface at higher magnification. Scale bar = 200 nm.

Pachebat JA, Kadekoppala M, Grainger M, Dluzewski AR, Gunaratne RS, Scott-Finnigan TJ, Ogun SA, Ling IT, Bannister LH, Taylor HM, Mitchell GH, Holder AA. Extensive proteolytic processing of the malaria parasite merozoite surface protein 7 during biosynthesis and parasite release from erythrocytes. Mol Biochem Parasitol. 2007 51:59-69. Copyright Elsevier 2009.