PF3D7_1402300 26S proteasome regulatory subunit RPN6

Intraerythrocytic expression of Rpn6 and Rpn6ΔN. (A) Stages of the erythrocytic life cycle of Rpn6-RFA (Upper) and Rpn6ΔN-RFA (Lower) parasites observed by live fluorescence microscopy. Images left to right are phase, DAPI, GFP, and fluorescence merge. RFA - regulatable, fluorescent affinity (RFA) tag. Rpn6 and Rpn6ΔN were expressed in all intraerythrocytic life cycle stages and were located in the cytoplasm. Rpn6ΔN-RFA indicates deletion of the asparagine repeat. It is shown that the asparagine repeat is dispensable for protein expression, stability, and function.

Muralidharan V, Oksman A, Iwamoto M, Wandless TJ, Goldberg DE. Asparagine repeat function in a Plasmodium falciparum protein assessed via a regulatable fluorescent affinity tag. Proc Natl Acad Sci U S A. 2011 108:4411-6

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