A trophozoite exhibiting GFP fluorescence in the food vacuole (arrowhead) and in a perinuclear ring (arrow). Fluorescence from the nuclear stain Hoechst 33342 is pseudocolored red. (B) A trophozoite with a fluorescent food vacuole (arrowhead) and a bright fluorescent spot (arrow) that lies at the periphery of the parasite. (C) A mature parasite displaying a large fluorescent food vacuole. Note the absence of fluorescence outside of the food vacuole. Bar, 2 mm.
Klemba et al., 2004. Originally published in The Journal of Cell Biology. doi:10.1083/jcb200307147. Klemba M, Beatty W, Gluzman I, Goldberg DE. Trafficking of plasmepsin II to the food vacuole of the malaria parasite Plasmodium falciparum. J Cell Biol. 2004 Jan 5;164(1):47-56.