PF3D7_1420400 glycine--tRNA ligase

Epitope-tagging of Plasmodium falciparum tRNAThr, tRNAGly and tRNAAla. The single, chromosomal copies of PfThrRS, PfGlyRS and PfAlaRS were tagged with a triple hemagglutinin through transfection and 3’ replacement. Immunfluorescence assays show that the majority of each of these epitope tagged proteins is localized to the parasite cytosol. An early trophozoite and a schizont stage parasite are shown for each parasite line. Parasites were labelled with anti-HA and anti-acyl carrier protein antibodies and DAPI. Bright field panels show the parasite (P) residing in its host erythrocyte (E). Some epitope tagged protein colocalises with the apicoplast marker ACP, but the dominant signal from the cytosolic HA-tagged protein in these parasites makes it unclear whether this is specific colocalisation or signal from adjacent protein. The very small size of the apicoplast (~200 nm diameter) makes this question difficult to resolve by light microscopy under these conditions.

Jackson KE, Pham JS, Kwek M, De Silva NS, Allen SM, Goodman CD, McFadden GI, Ribas de Pouplana L, Ralph SA. Dual targeting of aminoacyl-tRNA synthetases to the apicoplast and cytosol in Plasmodium falciparum. Int J Parasitol. 2012 42:177-86.

Other associated proteins

PFID Formal Annotation
PF3D7_1126000 threonine--tRNA ligase
PF3D7_1367700 alanine--tRNA ligase