Immunofluorescent anti-HA detection in fixed smears of erythrocytic stages of the PfPKG-HA-3A clone. Representative images of (i) a ring stage parasite, (ii) three early trophozoites, (iii) an early schizont, (iv, v) late schizonts (approximate hours post invasion: (i) 4–10, (ii) 20–26, (iii) 33–39, (iv, v) 45–48) and (vi) a stage III gametocyte are shown together with bright field images (first column) and parasite nuclei stained with DAPI (second column). Bars ,5 mm. epitope-tagged PfPKG was detected most strongly in late blood stage parasites. A diffuse pattern of relatively low level PfPKG expression was detected in ring and early trophozoite stages and gametocytes. Very similar results were obtained using PfPKG-PTP-1A clones, as well as the parental 3D7 parasites in conjunction with the commercial human PKG-I antibody. PfPKG is primarily found in the cytosol.
Hopp CS, Flueck C, Solyakov L, Tobin A, Baker DA. Spatiotemporal and Functional Characterisation of the Plasmodium falciparum cGMP-Dependent Protein Kinase. PLoS One. 2012;7(11):e48206.