Subcellular location of PfPKG in mature schizonts. Dual immunofluorescent detection of PfPKG-HA in fixed smears of early and late schizonts of the PfPKG-HA-3A clone together with (A) GAPDH; (B) BiP; (C) PMV; (D) Rab11A and (E) GAP45. Representative images are shown for each antibody, together with bright field images (first column) and parasite nuclei stained with DAPI (in the merged image). Bars ,5 mm. Significant overlap between the PKG-HA and GAPDH in early and late blood stage schizonts (A), both are in the cytosol. PfPKG-HA also partially overlapped with that of the ER membrane protein PMV and that of the ER lumen marker PfBiP (B and C) in both early and late schizonts. Rab11A is thought to be involved in vesicle trafficking and localises to the rhoptries and the inner membrane complex (IMC) at the apical end of the merozoite. In mature schizonts, the location of PKG-HA appeared to be largely distinct from that of Rab11A (D). PKG-HA appeared to be absent from the IMC in segmented schizonts, since it did not colocalise with the IMC marker, the glideosome associated protein 45. Hopp CS, Flueck C, Solyakov L, Tobin A, Baker DA. Spatiotemporal and Functional Characterisation of the Plasmodium falciparum cGMP-Dependent Protein Kinase. PLoS One. 2012;7(11):e48206.
Other associated proteins
|PF3D7_1222700||glideosome-associated protein 45|
|PF3D7_1320600||ras-related protein Rab-11A|
|PF3D7_1323500||PEXEL protease plasmepsin V|
|PF3D7_1436600||cGMP-dependent protein kinase|