Fluorescence micrographs of schizonts. The primary antibody was a monoclonal antibody that reacts with the P. falciparum rhoptry neck protein PfRON4 (green). Nuclei were counterstained with 0.5 g/ml DAPI (blue). Scale bar, 5 mm. (B) Fluorescence micrographs of schizonts. The primary antibodies were a monoclonal antibody that reacts with P. falciparum MSP1 (top row) and a polyclonal antibody that reacts with PfPKG (bottom row) (both green). Nuclei were counterstained with 0.5 g/ml DAPI (blue). The staining pattern observed throughout the merozoites suggests a predominantly cytosolic localization.

Taylor HM, McRobert L, Grainger M, Sicard A, Dluzewski AR, Hopp CS, Holder AA, Baker DA. The malaria parasite cyclic GMP-dependent protein kinase plays a central role in blood-stage schizogony. Eukaryot Cell. 2010 9:37-45.